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Burkin, A. A./ G. P. Kononenko/ T. Y. Tolpysheva 2013: Enzyme immunoassay of usnic acid in lichens. - Applied Biochemistry and Microbiology 49(3): 315-321. [RLL List # 232 / Rec.# 34875]
Abstract: An enzyme immunoassay for usnic acid in lichens was developed, the sensitivity of which was 0. 1 ?g/g of air-dried material (0. 00001%). Polyclonal rabbit antibodies against bovine serum albumin conjugated to (+)-usnic acid under the conditions of formaldehyde condensation made it possible to determine the analyzed substance in solutions at concentrations from 1 ng/mL when it interacts with immobilized gelatin conjugate homologous in the binding mode. Usnic acid in 2-26600 ?g/g (0. 0002-2. 6%) amounts was found in all 236 studied samples of lichens belonging to 53 species and 8 families. © 2013 Pleiades Publishing, Ltd.

URL: http://dx.doi.org/10.1134/S000368381303006X

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